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Background  Adhesion of  Mycoplasma pneumoniae  ( M. pneumoniae ) to host epithelial cells requires several adhesin proteins like P1, P30 and P116. Among these proteins, P1 protein has been inedited as one of the major adhesin and immunogenic protein present on the attachment organelle of  M. pneumoniae . In the present study, we scanned the entire sequence of  M. pneumoniae  P1 protein to identify the immunodominant and cytadherence region(s).  M. pneumoniae  P1 gene was synthesized in four segments replacing all the UGA codons to UGG codons. Each of the four purified P1 protein fragment was analyzed for its immunogenicity with anti- M. pneumoniae  M129 antibodies (Pab M129) and sera of  M. pneumoniae  infected patients by western blotting and ELISA. Antibodies were produced against all the P1 protein fragments and these antibodies were used for  M. pneumoniae  adhesion,  M. pneumoniae  adhesion inhibition and  M. pneumoniae  surface exposure assays using HEp-2 cells lines.    Results  Our results show that the immunodominant regions are distributed throughout the entire length of P1 protein, while only the N- and C- terminal region(s) of P1 protein are surface exposed and block cytadhesion to HEp-2 cells, while antibodies to two middle fragments failed to block cytadhesion.    Conclusions  These results have important implications in designing strategies to block the attachment of  M. pneumoniae  to epithelial cells, thus preventing the development of atypical pneumonia.

Delineation of immunodominant and cytadherence segment(s) of Mycoplasma pneumoniae P1 gene