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Background  The bacterial surface protein internalin (InlA) is a major virulence factor of the food-born pathogen  Listeria monocytogenes . It plays a critical role in the bacteria crossing the host intestinal barrier by a species-specific interaction with the cell adhesion molecule E-cadherin. In mice, the interaction of InlA with murine E-cadherin is impaired due to sequence-specific binding incompatibilities. We have previously used the approach of ‘murinisation’ to establish an oral listeriosis infection model in mice by exchanging two amino acid residues in InlA. This dramatically increases binding to mouse E-cadherin. In the present study, we have used bioluminescent murinised and non-murinised  Listeria  strains to examine the spatiotemporal dissemination of  Listeria  in four diverse mouse genetic backgrounds after oral inoculation.    Results  The murinised  Listeria monocytogenes  strain showed enhanced invasiveness and induced more severe infections in all four investigated mouse inbred strains compared to the non-murinised  Listeria  strain. We identified C57BL/6J mice as being most resistant to orally acquired listeriosis whereas C3HeB/FeJ, A/J and BALB/cJ mice were found to be most susceptible to infection. This was reflected in faster kinetics of  Listeria  dissemination, higher bacterial loads in internal organs, and elevated serum levels of IL-6, IFN-γ, TNF-α and CCL2 in the susceptible strains as compared to the resistant C57BL/6J strain. Importantly, murinisation of InlA did not cause enhanced invasion of  Listeria monocytogenes  into the brain.    Conclusion  Murinised  Listeria  are able to efficiently cross the intestinal barrier in mice from diverse genetic backgrounds. However, expression of murinized InlA does not enhance listerial brain invasion suggesting that crossing of the blood brain barrier and crossing of the intestinal epithelium are achieved by  Listeria monocytogenes  through different molecular mechanisms.

Influence of internalin a murinisation on host resistance to orally acquired listeriosis in mice