English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Methicillin resistance determinant  mecA  is generally transferred by SCC mec  elements. However, the  mecA  gene might not be carried by a SCC mec  in a  Staphylococcus haemolyticus  clinical isolate, WCH1, as no cassette chromosome recombinase genes were detected. Therefore, the genetic context of  mecA  in WCH1 was investigated.    Results  A 40-kb region containing  mecA  was obtained from WCH1, bounded by orfX at one end and several orfs of  S. haemolyticus  core chromosome at the other. This 40-kb region was very complex in structure with multiple genetic components that appeared to have different origins. For instance, the 3.7-kb structure adjacent to orfX was almost identical to that on the chromosome of  Staphylococcus epidermidis  RP62a but was absent from  S. haemolyticus  JCSC1435. Terminal inverted repeats of SCC were found but no  ccr  genes could be detected.  mecA  was bracketed by two copies of IS 431 , which was flanked by 8-bp direct target repeat sequence (DR).    Conclusions  The presence of 8-bp DR suggests that the two copies of IS 431  might have formed a composite transposon for mobilizing  mecA . This finding is of significance as multiple copies of IS 431  are commonly present in the contexts of  mecA , which might have the potential to form various composite transposons that could mediate the mobilization of  mecA . This study also provides an explanation for the absence of  ccr  in some staphylococci isolates carrying  mecA .

Characterization of a complex context containing mecA but lacking genes encoding cassette chromosome recombinases in Staphylococcus haemolyticus