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Background  Emergence of multidrug resistance in  Enterobacteriaceae  limits the selection of antimicrobials for treatment of infectious diseases. Identification of NDM-1 makes more difficulty in treating multidrug-resistant  Enterobacteriaceae  infections. Carbapenem-resistant  Escherichia coli  clinical isolates from a tertiary hospital in Wenzhou, east China, were investigated for NDM-1 production.    Results  The two tested isolates were negative for modified Hodge test, but positive for a double-disc synergy test used for detecting metallo-β-lactamase production.  E. coli  WZ33 and WZ51 exhibited discrepant-level resistance to most clinically frequent used antimicrobials, but still susceptible to trimethoprim/sulfamethoxazole, amikacin, fosfomycin, tigecycline and polymyxin B.  E. coli  WZ33 and WZ51 were positive for  bla  NDM-1  determined by PCR and DNA sequencing. Other than  bla  NDM-1 ,  E. coli  WZ33 also harbored  bla  CTX-M-14  and  bla  CMY-42 , while  E. coli  WZ51 simultaneously harbored  bla  SHV-12,  bla  CTX-M-14  and  bla  CMY-42 . Carbapenem resistance for  E. coli  WZ51 and WZ33 could not be transferred to  E. coli  recipients through conjugation, but could be transferred to  E. coli  recipients by chemical transformation. The EcoR1-digested DNA pattern of plasmids from the transformant of  E. coli  WZ51 was different from that of  E. coli  WZ51. MLST showed that  E. coli  WZ33 and WZ51 belonged to an animal-associated clone (ST167).    Conclusion  The present study is the first report of  bla  NDM-1  carriage in  E. coli  ST167 isolates and coexistence of  bla  NDM-1  and  bla  CMY-42  in same isolate. Systemic surveillance should focus on the dissemination of  bla  NDM-1  among Enterobacteriaceae, especially  E. coli  ST167 clone associated with animal infection.

First identification of coexistence of blaNDM-1 and blaCMY-42among Escherichia coli ST167 clinical isolates