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Background  The efficacy of entomopathogenic fungi in pest control is mainly affected by various adverse environmental factors, such as heat shock and UV-B radiation, and by responses of the host insect, such as oxidative stress, osmotic stress and fever. In this study, an adenylate cyclase gene ( MaAC ) was cloned from the locust-specific entomopathogenic fungus,  Metarhizium acridum , which is homologous to various fungal adenylate cyclase genes. RNA silencing was adapted to analyze the role of  MaAC  in virulence and tolerance to adverse environmental and host insect factors.    Results  Compared with the wild type, the vegetative growth of the RNAi mutant was decreased in PD (potato dextrose medium), Czapek-dox and PDA plates, respectively, demonstrating that  MaAC  affected vegetative growth. The cAMP levels were also reduced in PD liquid culture, and exogenous cAMP restored the growth of RNAi mutants. These findings suggested that  MaAC  is involved in cAMP synthesis. The knockdown of  MaAC  by RNAi led to a reduction in virulence after injection or topical inoculation. Furthermore, the RNAi mutant grew much slower than the wild type in the haemolymph of locust  in vitro  and  in vivo , thus demonstrating that  MaAC  affects the virulence of  M. acridum  via fungal growth inside the host locust. A plate assay indicated that the tolerances of the  MaAC  RNAi mutant under oxidative stress, osmotic stress, heat shock and UV-B radiation was decreased compared with the wild type.    Conclusion   MaAC  is required for virulence and tolerance to oxidative stress, osmotic stress, heat shock and UV-B radiation.  MaAC  affects fungal virulence via vegetative growth inside the insect and tolerance against oxidative stress, osmotic stress and locust fever.

The adenylate cyclase gene MaAC is required for virulence and multi-stress tolerance of Metarhizium acridum