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Background  The PII protein family comprises homotrimeric proteins which act as transducers of the cellular nitrogen and carbon status in prokaryotes and plants. In  Herbaspirillum seropedicae , two PII-like proteins (GlnB and GlnK), encoded by the genes  glnB  and  glnK , were identified. The  glnB  gene is monocistronic and its expression is constitutive, while  glnK  is located in the  nlmAglnKamtB  operon and is expressed under nitrogen-limiting conditions.    Results  In order to determine the involvement of the  H. seropedicae glnB  and  glnK  gene products in nitrogen fixation, a series of mutant strains were constructed and characterized. The  glnK -   mutants were deficient in nitrogen fixation and they were complemented by plasmids expressing the GlnK protein or an N-truncated form of NifA. The nitrogenase post-translational control by ammonium was studied and the results showed that the  glnK  mutant is partially defective in nitrogenase inactivation upon addition of ammonium while the  glnB  mutant has a wild-type phenotype.    Conclusions  Our results indicate that GlnK is mainly responsible for NifA activity regulation and ammonium-dependent post-translational regulation of nitrogenase in  H. seropedicae .

Role of PII proteins in nitrogen fixation control of Herbaspirillum seropedicae strain SmR1