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Background  Surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF-MS) was applied to analyze the protein profiles in both somatic and metabolic extracts of  Aspergillus  species. The study was carried out on some  Aspergillus  species within the  Fumigati  section ( Aspergillus fumigatus  wild-types and natural abnormally pigmented mutants, and  Aspergillus lentulus ). The aim was to validate whether mass spectrometry protein profiles can be used as specific signatures to discriminate different  Aspergillus  species or even mutants within the same species.    Results  The growth conditions and the SELDI-TOF parameters were determined to generate characteristic protein profiles of somatic and metabolic extracts of  Aspergillus fumigatus  strains using five different ProteinChips ® , eight growth conditions combining two temperatures, two media and two oxygenation conditions. Nine strains were investigated: three wild-types and four natural abnormally pigmented mutant strains of  A. fumigatus  and two strains of  A. lentulus . A total of 242 fungal extracts were prepared. The spectra obtained are protein signatures linked to the physiological states of fungal strains depending on culture conditions. The best resolutions were obtained using the chromatographic surfaces CM10, NP20 and H50 with fractions of fungi grown on modified Sabouraud medium at 37°C in static condition. Under these conditions, the SELDI-TOF analysis allowed  A. fumigatus  and  A. lentulus  strains to be grouped into distinct clusters.    Conclusions  SELDI-TOF analysis distinguishes  A. fumigatus  from  A. lentulus  strains and moreover, permits separate clusters of natural abnormally pigmented  A. fumigatus  strains to be obtained. In addition, this methodology allowed us to point out fungal components specifically produced by a wild-type strain or natural mutants. It offers attractive potential for further studies of the  Aspergillus  biology or pathogenesis.

bmc microbiology

Comparative proteomic profiles of Aspergillus fumigatus and Aspergillus lentulus strains by surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF-MS)