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Background  Sirodesmin PL is a secondary metabolite toxin made by the ascomycetous plant pathogen,  Leptosphaeria maculans . The sirodesmin biosynthetic genes are clustered in the genome. The key genes are a non-ribosomal peptide synthetase,  sirP , and a pathway-specific transcription factor,  sirZ . Little is known about regulation of sirodesmin production.    Results  Genes involved in regulation of sirodesmin PL in  L. maculans  have been identified. Two hundred random insertional T-DNA mutants were screened with an antibacterial assay for ones producing low levels of sirodesmin PL. Three such mutants were isolated and each transcribed  sirZ  at very low levels. One of the affected genes had high sequence similarity to  Aspergillus fumigatus cpcA , which regulates the cross-pathway control system in response to amino acid availability. This gene was silenced in  L. maculans  and the resultant mutant characterised. When amino acid starvation was artificially-induced by addition of 3-aminotriazole for 5 h, transcript levels of  sirP  and  sirZ  did not change in the wild type. In contrast, levels of  sirP  and  sirZ  transcripts increased in the silenced  cpcA  mutant. After prolonged amino acid starvation the silenced  cpcA  mutant produced much higher amounts of sirodesmin PL than the wild type.    Conclusions  Production of sirodesmin PL in  L. maculans  is regulated by the cross pathway control gene,  cpcA , either directly or indirectly via the pathway-specific transcription factor,  sirZ .

The cross-pathway control system regulates production of the secondary metabolite toxin, sirodesmin PL, in the ascomycete, Leptosphaeria maculans