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Background   sabR  is a pleiotropic regulatory gene which has been shown to positively regulate the nikkomycin biosynthesis and negatively affect the sporulation of  Streptomyces ansochromogenes . In this study, we investigate the mechanism of SabR on modulating nikkomycin production in  Streptomyces ansochromogenes .    Results  The transcription start point of  sabR  was determined by high-resolution S1 nuclease mapping and localized at the nucleotide T at position 37 bp upstream of the potential  sabR  translation start codon (GTG). Disruption of  sabR  enhanced its own transcription, but retarded the nikkomycin production. Over-expression of  sabR  enhanced nikkomycin biosynthesis in  Streptomyces ansochromogenes . EMSA analysis showed that SabR bound to the upstream region of  sanG , but it did not bind to the upstream region of its encoding gene ( sabR ),  sanF  and the intergenic region between  sanN  and  sanO . DNase 1 footprinting assays showed that the SabR-binding site upstream of  sanG  was 5'-CTTTAAGTCACCTGGCTCATTCGCGTTCGCCCAGCT-3' which was designated as SARE. Deletion of SARE resulted in the delay of nikkomycin production that was similar to that of  sabR  disruption mutant.    Conclusions  These results indicated that SabR modulated nikkomycin biosynthesis as an enhancer via interaction with the promoter region of  sanG , and expanded our understanding about regulatory cascade in nikkomycin biosynthesis.

bmc microbiology

SabR enhances nikkomycin production via regulating the transcriptional level of sanG, a pathway-specific regulatory gene in Streptomyces ansochromogenes