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Background  The  MP65  gene of  Candida albicans  (orf19.1779) encodes a putative β-glucanase mannoprotein of 65 kDa, which plays a main role in a host-fungus relationship, morphogenesis and pathogenicity. In this study, we performed an extensive analysis of a  mp65Δ  mutant to assess the role of this protein in cell wall integrity, adherence to epithelial cells and biofilm formation.    Results  The  mp65Δ  mutant showed a high sensitivity to a range of cell wall-perturbing and degrading agents, especially Congo red, which induced morphological changes such as swelling, clumping and formation of hyphae. The  mp65Δ  mutant showed an activation of two MAPKs (Mkc1p and Cek1p), a high level of expression of two stress-related genes (DDR48 and  SOD5 ), and a modulated expression of β-glucan epitopes, but no gross changes in cell wall polysaccharide composition. Interestingly, the  mp65Δ  mutant displayed a marked reduction in adhesion to BEC and Caco-2 cells and severe defects in biofilm formation when compared to the wild type. All of the mentioned properties were totally or partially recovered in a revertant strain, demonstrating the specificity of gene deletion.    Conclusions  We demonstrate that the  MP65  gene of  Candida albicans  plays a significant role in maintaining cell wall integrity, as well as in adherence to epithelia and biofilm formation, which are major virulence attributes of this fungus.

The MP65 gene is required for cell wall integrity, adherence to epithelial cells and biofilm formation in Candida albicans