English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  In recent years genome sequencing has been used to characterize new bacterial species, a method of analysis available as a result of improved methodology and reduced cost. Included in a constantly expanding list of  Vibrio  species are several that have been reclassified as novel members of the  Vibrionaceae . The description of two putative new  Vibrio  species,  Vibrio  sp. RC341 and  Vibrio  sp. RC586 for which we propose the names  V. metecus  and  V. parilis , respectively, previously characterized as non-toxigenic environmental variants of  V. cholerae  is presented in this study.    Results  Based on results of whole-genome average nucleotide identity (ANI), average amino acid identity (AAI),  rpoB  similarity, MLSA, and phylogenetic analysis, the new species are concluded to be phylogenetically closely related to  V. cholerae  and  V. mimicus .  Vibrio  sp. RC341 and  Vibrio  sp. RC586 demonstrate features characteristic of  V. cholerae  and  V. mimicus , respectively, on differential and selective media, but their genomes show a 12 to 15% divergence (88 to 85% ANI and 92 to 91% AAI) compared to the sequences of  V. cholerae  and  V. mimicus  genomes (ANI <95% and AAI <96% indicative of separate species).  Vibrio  sp. RC341 and  Vibrio  sp. RC586 share 2104 ORFs (59%) and 2058 ORFs (56%) with the published core genome of  V. cholerae  and 2956 (82%) and 3048 ORFs (84%) with  V. mimicus  MB-451, respectively. The novel species share 2926 ORFs with each other (81%  Vibrio  sp. RC341 and 81%  Vibrio  sp. RC586). Virulence-associated factors and genomic islands of  V. cholerae  and  V. mimicus , including VSP-I and II, were found in these environmental  Vibrio  spp.    Conclusions  Results of this analysis demonstrate these two environmental vibrios, previously characterized as variant  V. cholerae  strains, are new species which have evolved from ancestral lineages of the  V. cholerae  and  V. mimicus  clade. The presence of conserved integration loci for genomic islands as well as evidence of horizontal gene transfer between these two new species,  V. cholerae , and  V. mimicus  suggests genomic islands and virulence factors are transferred between these species.

Comparative genomic analysis reveals evidence of two novel Vibrio species closely related to V. cholerae