English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  We have recently found that  Pseudomonas putida  deficient in ColRS two-component system is sensitive to phenol and displays a serious defect on solid glucose medium where subpopulation of bacteria lyses. The latter phenotype is significantly enhanced by the presence of phenol in growth medium. Here, we focused on identification of factors affecting phenol tolerance of the  colR -deficient  P. putida .    Results  By using transposon mutagenesis approach we identified a set of phenol-tolerant derivatives of  colR -deficient strain. Surprisingly, half of independent phenol tolerant clones possessed miniTn5 insertion in the  ttgABC  operon. However, though inactivation of TtgABC efflux pump significantly enhanced phenol tolerance, it did not affect phenol-enhanced autolysis of the  colR  mutant on glucose medium indicating that phenol- and glucose-caused stresses experienced by the  colR -deficient  P. putida  are not coupled. Inactivation of TtgABC pump significantly increased the phenol tolerance of the wild-type  P. putida  as well. Comparison of phenol tolerance of growing  versus  starving bacteria revealed that both ColRS and TtgABC systems affect phenol tolerance only under growth conditions and not under starvation. Flow cytometry analysis showed that phenol strongly inhibited cell division and to some extent also caused cell membrane permeabilization to propidium iodide. Single cell analysis of populations of the  ttgC-  and  colRttgC- deficient strains revealed that their membrane permeabilization by phenol resembles that of the wild-type and the  colR  mutant, respectively. However, cell division of  P. putida  with inactivated TtgABC pump seemed to be less sensitive to phenol than that of the parental strain. At the same time, cell division appeared to be more inhibited in the  colR -mutant strain than in the wild-type  P. putida .    Conclusions  ColRS signal system and TtgABC efflux pump are involved in the phenol tolerance of  P. putida . However, as they affect phenol tolerance of growing bacteria only, this indicates that they participate in the regulation of processes which are active during the growth and/or cell division. Single cell analysis data indicated that the cell division step of cell cycle is particularly sensitive to the toxic effect of phenol and its inhibition can be considered as an adaptive response under conditions of phenol stress.

The impact of ColRS two-component system and TtgABC efflux pump on phenol tolerance of Pseudomonas putida becomes evident only in growing bacteria