English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  The genomes of  Streptomyces coelicolor  and  Streptomyces lividans  bear a considerable degree of synteny. While  S. coelicolor  is the model streptomycete for studying antibiotic synthesis and differentiation,  S. lividans  is almost exclusively considered as the preferred host, among actinomycetes, for cloning and expression of exogenous DNA. We used whole genome microarrays as a comparative genomics tool for identifying the subtle differences between these two chromosomes.    Results  We identified five large  S. coelicolor  genomic islands (larger than 25 kb) and 18 smaller islets absent in  S. lividans  chromosome. Many of these regions show anomalous GC bias and codon usage patterns. Six of them are in close vicinity of tRNA genes while nine are flanked with near perfect repeat sequences indicating that these are probable recent evolutionary acquisitions into  S. coelicolor . Embedded within these segments are at least four DNA methylases and two probable methyl-sensing restriction endonucleases. Comparison with  S. coelicolor  transcriptome and proteome data revealed that some of the missing genes are active during the course of growth and differentiation in  S. coelicolor . In particular, a pair of methylmalonyl CoA mutase ( mcm ) genes involved in polyketide precursor biosynthesis, an acyl-CoA dehydrogenase implicated in timing of actinorhodin synthesis and  bldB , a developmentally significant regulator whose mutation causes complete abrogation of antibiotic synthesis belong to this category.    Conclusion  Our findings provide tangible hints for elucidating the genetic basis of important phenotypic differences between these two streptomycetes. Importantly, absence of certain genes in  S. lividans  identified here could potentially explain the relative ease of DNA transformations and the conditional lack of actinorhodin synthesis in  S. lividans .

Comparative genomic hybridizations reveal absence of large Streptomyces coelicolor genomic islands in Streptomyces lividans