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Materials and methods This study was designed to investigate the involvement of WT1 gene mutation in the development of Acute Myeloid Leukemia in Sudan. The study involved 51 patients and 75 healthy controls. Genomic DNA was isolated from peripheral blood leukocytes using salting out method. PCR amplification of the target sequence (214 bp) within exon7 of WT1 gene was carried out. The known A®G transition mutation that destroys an AflIII restriction enzyme recognition site was detected by RFLP analysis.

A single nucleotide polymorphism in Wilms’ tumor 1 gene and the risk of acute myeloid leukemia in Sudan