English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Polar overdominance at the ovine callipyge ( CLPG ) locus involves the post-transcriptional  trans -inhibition of  DLK1  in skeletal muscle of  CLPG/CLPG  sheep. The abundant maternally expressed microRNAs (miRNAs) mapping to the imprinted  DLK1-GTL2  domain are prime candidate mediators of this  trans -effect.    Results  We have tested the affinity of 121 miRNAs processed from this locus for  DLK1  by co-transfecting COS1 cells with a vector expressing the full-length ovine DLK1 with corresponding mimic miRNAs. None of the tested miRNAs was able to down regulate DLK1 to the extent observed  in vivo .    Conclusions  This suggests that other factors, with or without these miRNAs, are involved in mediating the observed  trans -effect.    Electronic supplementary material  The online version of this article (doi:10.1186/1471-2164-15-944) contains supplementary material, which is available to authorized users.

Experimental evaluation does not reveal a direct effect of microRNA from the callipyge locus on DLK1 expression