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Background  Werner Syndrome (WS) is a rare disorder characterized by the premature onset of a number of age-related diseases. The gene responsible for WS encodes a DNA helicase/exonuclease protein believed to affect different aspects of transcription, replication, and DNA repair.  Caenorhabditis elegans (C. elegans)  with a nonfunctional  wrn-1  DNA helicase ortholog also exhibits a shorter life span, which can be rescued by vitamin C. In this study, we analyzed the impact of a mutation in the  wrn-1  gene and the dietary supplementation of vitamin C on the global mRNA expression of the whole  C. elegans  by the RNA-seq technology.    Results  Vitamin C increased the mean life span of the  wrn-1 ( gk99 ) mutant and the N2 wild type strains at 25°C. However, the alteration of gene expression by vitamin C is different between  wrn-1 ( gk99 ) and wild type strains. We observed alteration in the expression of 1522 genes in  wrn-1 ( gk99 ) worms compared to wild type animals. Such genes significantly affected the metabolism of lipid, cellular ketone, organic acid, and carboxylic acids. Vitamin C, in return, altered the expression of genes in  wrn-1 ( gk99 ) worms involved in locomotion and anatomical structure development. Proteolysis was the only biological process significantly affected by vitamin C in wild type worms.    Conclusions  Expression profiling of  wrn-1 ( gk99 ) worms revealed a very different response to the addition of vitamin C compared to wild type worms. Finally, vitamin C extended the life span of  wrn-1 ( gk99 ) animals by altering biological processes involved mainly in locomotion and anatomical structure development.    Electronic supplementary material  The online version of this article (doi:10.1186/1471-2164-15-940) contains supplementary material, which is available to authorized users.

bmc genomics

Expression profile of Caenorhabditis elegans mutant for the Werner syndrome gene ortholog reveals the impact of vitamin C on development to increase life span