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Background  The current knowledge of eukaryote signalling originates from phenotypically diverse organisms. There is a pressing need to identify conserved signalling components among eukaryotes, which will lead to the transfer of knowledge across kingdoms. Two useful properties of a eukaryote model for signalling are (1) reduced signalling complexity, and (2) conservation of signalling components. The alga  Ostreococcus tauri  is described as the smallest free-living eukaryote. With less than 8,000 genes, it represents a highly constrained genomic palette.    Results  Our survey revealed 133 protein kinases and 34 protein phosphatases (1.7% and 0.4% of the proteome). We conducted phosphoproteomic experiments and constructed domain structures and phylogenies for the catalytic protein-kinases. For each of the major kinases families we review the completeness and divergence of  O. tauri  representatives in comparison to the well-studied kinomes of the laboratory models  Arabidopsis thaliana  and  Saccharomyces cerevisiae,  and of  Homo sapiens . Many kinase clades in  O. tauri  were reduced to a single member, in preference to the loss of family diversity, whereas TKL and ABC1 clades were expanded. We also identified kinases that have been lost in  A. thaliana  but retained in  O. tauri . For three, contrasting eukaryotic pathways – TOR, MAPK, and the circadian clock – we established the subset of conserved components and demonstrate conserved sites of substrate phosphorylation and kinase motifs.    Conclusions  We conclude that  O. tauri  satisfies our two central requirements. Several of its kinases are more closely related to  H. sapiens  orthologs than  S. cerevisiae  is to  H. sapiens.  The greatly reduced kinome of  O. tauri  is therefore a suitable model for signalling in free-living eukaryotes.    Electronic supplementary material  The online version of this article (doi:10.1186/1471-2164-15-640) contains supplementary material, which is available to authorized users.

The reduced kinome of Ostreococcus tauri: core eukaryotic signalling components in a tractable model species