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We explored the use of genotyping by sequencing (GBS) on a recombinant inbred line population (GPMx) derived from a cross between the two-rowed barley cultivar ‘Golden Promise’ ( ari-e.GP / Vrs1 ) and the six-rowed cultivar ‘Morex’ ( Ari-e/vrs1 ) to map plant height. We identified three Quantitative Trait Loci (QTL), the first in a region encompassing the spike architecture gene  Vrs1  on chromosome 2H, the second in an uncharacterised centromeric region on chromosome 3H, and the third in a region of chromosome 5H coinciding with the previously described dwarfing gene  Breviaristatum-e  ( Ari-e) .    Background  Barley cultivars in North-western Europe largely contain either of two dwarfing genes;  Denso  on chromosome 3H, a presumed ortholog of the rice green revolution gene  OsSd1,  or  Breviaristatum-e  ( ari-e)  on chromosome 5H. A recessive mutant allele of the latter gene,  ari-e.GP , was introduced into cultivation via the  cv.  ‘Golden Promise’ that was a favourite of the Scottish malt whisky industry for many years and is still used in agriculture today.    Results  Using GBS mapping data and phenotypic measurements we show that  ari-e.GP  maps to a small genetic interval on chromosome 5H and that alternative alleles at a region encompassing  Vrs1  on 2H along with a region on chromosome 3H also influence plant height. The location of  Ari-e  is supported by analysis of near-isogenic lines containing different  ari-e  alleles. We explored use of the GBS to populate the region with sequence contigs from the recently released physically and genetically integrated barley genome sequence assembly as a step towards  Ari-e  gene identification.    Conclusions  GBS was an effective and relatively low-cost approach to rapidly construct a genetic map of the GPMx population that was suitable for genetic analysis of row type and height traits, allowing us to precisely position  ari-e.GP  on chromosome 5H. Mapping resolution was lower than we anticipated. We found the GBS data more complex to analyse than other data types but it did directly provide linked SNP markers for subsequent higher resolution genetic analysis.

An evaluation of genotyping by sequencing (GBS) to map the Breviaristatum-e (ari-e) locus in cultivated barley