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Background  ADA2 proteins, together with ADA3, SGF29 and GCN5 form the acetyltransferase module of GNAT-type histone acetyltransferase complexes. ADA2b is present in the SAGA complex, which plays roles in various chromatin-related processes via histone H3 modifications and by other mechanisms.    Results  In this report we present findings showing that during  Drosophila melanogaster  development two dADA2b isoforms (dADA2bS and dADA2bL) - which differ in their C-terminal domains - are expressed at various levels. Genetic complementation experiments indicate that dADA2bS alone can support development but cannot fully complement  dAda2b  mutations. In the presence of dADA2bS, the SAGA-specific histone H3 acetylation level is partially restored in  dAda2b  mutants. Comparison of whole transcriptome profiles of  dAda2b  null and  dAda2bS  transgene-carrier  dAda2b  null larvae indicates partial overlap between affected genes. mRNA levels corresponding to selected genes which are either up- or down-regulated in  dAda2b  mutants are altered by dADA2bS expression to different extents, ranging from complete restoration to wild type levels to no restoration at all. The short (dADA2bS) isoform of dADA2b seems to be more capable of restoring lost dSAGA functions that cause mRNA level up-regulation than those that lead to decreased mRNA levels.    Conclusions  The data presented here are in accord with results of genetic complementation experiments, and support the hypothesis that different isoforms of dADA2b contribute to the functional variations of dSAGA multiprotein HAT complexes.

Functional characterization and gene expression profiling of Drosophila melanogastershort dADA2b isoform-containing dSAGA complexes