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Background  The Australian sheep blowfly  Lucilia cuprina  (Wiedemann) (Diptera: Calliphoridae) is a destructive pest of the sheep, a model organism for insecticide resistance research, and a valuable tool for medical and forensic professionals. However, genomic information on  L. cuprina  is still sparse.    Results  We report here the construction of an embryonic and 2 larval cDNA libraries for  L. cuprina . A total of 29,816 expressed sequence tags (ESTs) were obtained and assembled into 7,464 unique clusters. The sequence collection captures a great diversity of genes, including those related to insecticide resistance (e.g., 12 cytochrome P450s, 2 glutathione S transferases, and 6 esterases). Compared to  Drosophila melanogaster , codon preference is different in 13 of the 18 amino acids encoded by redundant codons, reflecting the lower overall GC content in  L. cuprina . In addition, we demonstrated that the ESTs could be converted into informative gene markers by capitalizing on the known gene structures in the model organism  D. melanogaster . We successfully assigned 41 genes to their respective chromosomes in  L. cuprina . The relative locations of these loci revealed high but incomplete chromosomal synteny between  L. cuprina  and  D. melanogaster .    Conclusions  Our results represent the first major transcriptomic undertaking in  L. cuprina . These new genetic resources could be useful for the blowfly and insect research community.

Identification, analysis, and linkage mapping of expressed sequence tags from the Australian sheep blowfly