English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Transcriptome sequencing data has become an integral component of modern genetics, genomics and evolutionary biology. However, despite advances in the technologies of DNA sequencing, such data are lacking for many groups of living organisms, in particular, many plant taxa. We present here the results of transcriptome sequencing for two closely related plant species. These species,  Fagopyrum esculentum  and  F. tataricum , belong to the order Caryophyllales - a large group of flowering plants with uncertain evolutionary relationships.  F. esculentum  (common buckwheat) is also an important food crop. Despite these practical and evolutionary considerations  Fagopyrum  species have not been the subject of large-scale sequencing projects.    Results  Normalized cDNA corresponding to genes expressed in flowers and inflorescences of  F. esculentum  and  F. tataricum  was sequenced using the 454 pyrosequencing technology. This resulted in 267 (for  F. esculentum ) and 229 ( F. tataricum ) thousands of reads with average length of 341-349 nucleotides.  De novo  assembly of the reads produced about 25 thousands of contigs for each species, with 7.5-8.2× coverage. Comparative analysis of two transcriptomes demonstrated their overall similarity but also revealed genes that are presumably differentially expressed. Among them are retrotransposon genes and genes involved in sugar biosynthesis and metabolism. Thirteen single-copy genes were used for phylogenetic analysis; the resulting trees are largely consistent with those inferred from multigenic plastid datasets. The sister relationships of the Caryophyllales and asterids now gained high support from nuclear gene sequences.    Conclusions  454 transcriptome sequencing and  de novo  assembly was performed for two congeneric flowering plant species,  F. esculentum  and  F. tataricum . As a result, a large set of cDNA sequences that represent orthologs of known plant genes as well as potential new genes was generated.

De novo sequencing and characterization of floral transcriptome in two species of buckwheat (Fagopyrum)