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Background  The ribosomal protein SA (RPSA), previously named 37-kDa laminin receptor precursor/67-kDa laminin receptor (LRP/LR) is a multifunctional protein that plays a role in a number of pathological processes, such as cancer and prion diseases. In all investigated species,  RPSA  is a member of a multicopy gene family consisting of one full length functional gene and several pseudogenes. Therefore, for studies on RPSA related pathways/pathologies, it is important to characterize the whole family and to address the possible function of the other  RPSA  family members. The present work aims at deciphering the  RPSA  family in sheep.    Results  In addition to the full length functional ovine  RPSA  gene, 11 other members of this multicopy gene family, all processed pseudogenes, were identified. Comparison between the  RPSA  transcript and these pseudogenes shows a large variety in sequence identities ranging from 99% to 74%. Only one of the 11 pseudogenes, i.e.  RPSAP7 , shares the same open reading frame (ORF) of 295 amino acids with the  RPSA  gene, differing in only one amino acid. All members of the  RPSA  family were annotated by comparative mapping and fluorescence  in situ  hybridization (FISH) localization. Transcription was investigated in the cerebrum, cerebellum, spleen, muscle, lymph node, duodenum and blood, and transcripts were detected for 6 of the 11 pseudogenes in some of these tissues.    Conclusions  In the present work we have characterized the ovine  RPSA  family. Our results have revealed the existence of 11 ovine  RPSA  pseudogenes and provide new data on their structure and sequence. Such information will facilitate molecular studies of the functional  RPSA  gene taking into account the existence of these pseudogenes in the design of experiments. It remains to be investigated if the transcribed members are functional as regulatory non-coding RNA or as functional proteins.

Characterization of the ovine ribosomal protein SA gene and its pseudogenes