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Background  Proper patterning of the follicle cell epithelium over the egg chamber is essential for the  Drosophila  egg development. Differentiation of the epithelium into several distinct cell types along the anterior-posterior axis requires coordinated activities of multiple signaling pathways. Previously, we reported that  lethal(2)giant larvae  ( lgl ), a  Drosophila  tumor suppressor gene, is required in the follicle cells for the posterior follicle cell (PFC) fate induction at mid-oogenesis. Here we explore the role of another two tumor suppressor genes,  scribble  ( scrib ) and  discs large  ( dlg ), in the epithelial patterning.    Results  We found that removal of  scrib  or  dlg  function from the follicle cells at posterior terminal of the egg chamber causes a complete loss of the PFC fate. Aberrant specification and differentiation of the PFCs in the mosaic clones can be ascribed to defects in coordinated activation of the EGFR, JAK and Notch signaling pathways in the multilayered cells. Meanwhile, the clonal analysis revealed that loss-of-function mutations in  scrib/dlg  at the anterior domains result in a partially penetrant phenotype of defective induction of the stretched and centripetal cell fate, whereas specification of the border cell fate can still occur in the most anterior region of the mutant clones. Further, we showed that  scrib  genetically interacts with  dlg  in regulating posterior patterning of the epithelium.    Conclusion  In this study we provide evidence that  scrib  and  dlg  function differentially in anterior and posterior patterning of the follicular epithelium at oogenesis. Further genetic analysis indicates that  scrib  and  dlg  act in a common pathway to regulate PFC fate induction. This study may open another window for elucidating role of  scrib/dlg  in controlling epithelial polarity and cell proliferation during development.

Role of Scrib and Dlg in anterior-posterior patterning of the follicular epithelium during Drosophila oogenesis