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Background  To date, the earliest stage at which the orientation of the anterior-posterior axis in the mouse embryo is distinguishable by asymmetric gene expression is shortly after E5.5. At E5.5, prospective anterior markers are expressed at the distal tip of the embryo, whereas prospective posterior markers are expressed more proximally, close to the boundary with the extraembryonic region.    Results  To contribute to elucidating the mechanisms underlying the events involved in early patterning of the mouse embryo, we have carried out a microarray screen to identify novel genes that are differentially expressed between the distal and proximal parts of the E5.5 embryo. Secondary screening of resulting candidates by  in situ  hybridisation at E5.5 and E6.5 revealed novel expression patterns for known and previously uncharacterised genes, including  Peg10 ,  Ctsz1 ,  Cubilin ,  Jarid1b ,  Ndrg1 ,  Sfmbt2 ,  Gjb5 ,  Talia  and  Plet1 . The previously undescribed gene  Talia  and recently identified  Plet1  are expressed specifically in the distal-most part of the extraembryonic ectoderm, adjacent to the epiblast, and are therefore potential candidates for regulating early patterning events.  Talia  and the previously described gene  XE7  define a gene family highly conserved among metazoans and with a predicted protein structure suggestive of a post-transcriptional regulative function, whilst  Plet1  appears to be mammal-specific and of unknown function.    Conclusion  Our approach has allowed us to compare expression between dissected parts of the egg cylinder and has identified multiple genes with novel expression patterns at this developmental stage. These genes are potential candidates for regulating tissue interactions following implantation.

Novel gene expression patterns along the proximo-distal axis of the mouse embryo before gastrulation