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This study describes the development of a system that calculates the fluxes of a metabolic network. It integrates several open source software tools in order to simulate the behaviour of specific metabolic pathways using Flux Balance Analysis (FBA). This tool receives as input the stoichiometry, the thermodynamic and capacity constraints of the metabolic network, and also an objective function.

bmc bioinformatics

Integration of open source software tools for the in silico design of metabolic pathways using flux balance analysis – an application to the production of recombinant human proteins