English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Exposure to vanadium pentoxide (V 2 O 5 ) is a cause of occupational bronchitis. We evaluated gene expression profiles in cultured human lung fibroblasts exposed to V 2 O 5   in vitro  in order to identify candidate genes that could play a role in inflammation, fibrosis, and repair during the pathogenesis of V 2 O 5 -induced bronchitis.    Methods  Normal human lung fibroblasts were exposed to V 2 O 5  in a time course experiment. Gene expression was measured at various time points over a 24 hr period using the Affymetrix Human Genome U133A 2.0 Array. Selected genes that were significantly changed in the microarray experiment were validated by RT-PCR.    Results  V 2 O 5  altered more than 1,400 genes, of which ~300 were induced while >1,100 genes were suppressed. Gene ontology categories (GO) categories unique to induced genes included  inflammatory response  and  immune response , while GO catogories unique to suppressed genes included  ubiquitin cycle  and  cell cycle . A dozen genes were validated by RT-PCR, including growth factors ( HBEGF ,  VEGF ,  CTGF ), chemokines ( IL8 ,  CXCL9 ,  CXCL10 ), oxidative stress response genes ( SOD2 ,  PIPOX ,  OXR1 ), and DNA-binding proteins ( GAS1 ,  STAT1 ).    Conclusion  Our study identified a variety of genes that could play pivotal roles in inflammation, fibrosis and repair during V 2 O 5 -induced bronchitis. The induction of genes that mediate inflammation and immune responses, as well as suppression of genes involved in growth arrest appear to be important to the lung fibrotic reaction to V 2 O 5 .

Genomic analysis of human lung fibroblasts exposed to vanadium pentoxide to identify candidate genes for occupational bronchitis