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The Extradomain A from fibronectin (EDA) has an immunomodulatory role as fusion protein with viral and tumor antigens, but its effect when administered with bacteria has not been assessed. Here, we investigated the adjuvant effect of EDA in mice immunizations against  Salmonella enterica  subspecies  enterica  serovar Enteritidis ( Salmonella  Enteritidis). Since lipopolysaccharide (LPS) is a major virulence factor and the LPS O-polysaccharide (O-PS) is the immunodominant antigen in serological diagnostic tests,  Salmonella  mutants lacking O-PS (rough mutants) represent an interesting approach for developing new vaccines and diagnostic tests to differentiate infected and vaccinated animals (DIVA tests). Here, antigenic preparations (hot-saline extracts and formalin-inactivated bacterins) from two  Salmonella  Enteritidis rough mutants, carrying either intact (SEΔ waaL ) or deep-defective (SEΔ gal ) LPS-Core, were used in combination with EDA. Biotinylated bacterins, in particular SEΔ waaL  bacterin, decorated with EDAvidin (EDA and streptavidin fusion protein) improved the protection conferred by hot-saline or bacterins alone and prevented significantly the virulent infection at least to the levels of live attenuated rough mutants. These findings demonstrate the adjuvant effect of EDAvidin when administered with biotinylated bacterins from  Salmonella  Enteritidis lacking O-PS and the usefulness of BEDA-SEΔ waaL  as non-live vaccine in the mouse model.

The extradomain a of fibronectin enhances the efficacy of lipopolysaccharide defective Salmonella bacterins as vaccines in mice