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Methods We used a model of trigeminovascular nociception in Sprague Dawley rats that measures transmission of craniovascular nociception in the trigeminocervical complex (TCC) by the firing of TCC neurons evoked in response to electrical stimulation of afferents from the middle meningeal artery, its branches, and periarterial meninges (MMA). To determine whether GABA receptors in the NRM modulate this nociceptive transmission, and to characterize the modulation, we microinjected GABA, and GABAAand GABAB-receptor agonists and antagonists into the NRM.

GABAA receptors in the nucleus raphe magnus modulate firing of neurons in the trigeminocervical complex