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Separation of the polypeptide chains of hemoglobin and identification of an abnormal chain may be achieved in a few hours by electrophoresis on cellulose acetate in barbital buffer pH 8.6, with 6 M urea. The method is applicable to whole hemolysates to which a large amount of 2-mercaptoethanol has been added. It requires minimal preparative work.

Brief Report: Rapid Differentiation of Polypeptide Chains of Hemoglobin by Cellulose Acetate Electrophoresis of Hemolylsates