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Objectives The present study reports the multilocus genotyping of Giardia duodenalis isolates from cats maintained in breeding catteries in Japan and discusses their potential for zoonotic transmission. Methods A total of 41 faecal samples positive for Giardia -specific antigen were procured from cats maintained in five breeding catteries and subjected to PCR to amplify four gene loci, namely small subunit ribosomal RNA ( SSU rRNA ), glutamate dehydrogenase ( gdh ), beta-giardin ( bg ) and triose phosphate isomerase ( tpi  ). The PCR-amplified DNA fragments were sequenced to determine the G duodenalis genotypes (synonym for assemblages). Results The most commonly occurring single assemblage was assemblage F (68.3%; n = 28/41), followed by assemblage A (12.2%; n = 5/41) and assemblage C (2.4%; n = 1/41). The mixed assemblages were identified as follows: assemblages F and A (9.8%; n = 4/41), assemblages F and C (4.9%; n = 2/41) and assemblages C and D (2.4%; n = 1/41). Additional sub-genotyping of assemblage A isolates based on three of the sequenced loci ( gdh, bg and tpi  ) revealed that all eight isolates were identified as sub-assemblage AI and/or AII. Conclusions and relevance The present study is the first to report the detection of dog-adapted assemblages C and D in feline isolates from Japan. In addition, zoonotic sub-assemblage AI and human-adapted sub-assemblage AII were also identified. Thus, we concluded that the risk of transmission of G duodenalis from breeding cattery cats to humans is considerable and cannot be ignored

Multilocus genotyping of Giardia duodenalis isolates from breeding cattery cats in Japan