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Developing appropriate cell culturing techniques to populatescaffolds has become a great challenge in tissue engineering. This work describes theuse of spinner flask dynamic cell cultures to populate hydroxyapatite microcarriers forbone tissue engineering. The microcarriers were obtained through the emulsion of aself-setting aqueous α-tricalcium phosphate slurry in oil. After setting, hydroxyapatitemicrocarriers were obtained. The incorporation of gelatin in the liquid phase of theα-tricalcium phosphate slurry allowed obtaining hybridgelatin/hydroxyapatite-microcarriers. Initial cell attachment on the microcarriers wasstrongly influenced by the speed of the dynamic culture, achieving higher attachment atlow speed (40 r/min) as compared to high speed (80 r/min). Under moderate culture speeds(40 r/min), the number of cells present in the culture as well as the number ofmicrocarrier-containing cells considerably increased after 3 days, particularly in thegelatin-containing microcarriers. At longer culture times in dynamic culture,hydroxyapatite-containing microcarriers formed aggregates containing viable andextracellular matrix proteins, with a significantly higher number of cells compared tostatic cultures

Dynamic cell culture on calcium phosphate microcarriers for bone tissue engineering applications