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A new diphenyl ether derivative, named pestalotilide A (1), along with four known compounds (2-5) have been isolated from the organic extracts of an endophytic fungus Pestalotiposis guepinii. Their structures were elucidated on the basis of HR-ESI-MS, 1D and 2D NMR spectroscopic data analyses. These isolates were evaluated for cytotoxicity against three cancer cell lines: Hela, HCT116 and A549; pestalotilide A exhibited weak in vitro anti-proliferative activities against the tested cell lines, with IC 50  values of 23.8, 18.4 and 35.2 μM, respectively.

Pestalotilide A, a New Cytotoxic Diphenyl Ether Derivative from Pestalotiposis guepinii