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Flavonoids (in particular unsubstituted B ring flavanones) in Eucalyptus foliage play an important role in mediating animal plant interactions, and there is a need for methods to analyse the diverse profiles found in leaves. A simple, high-performance liquid chromatographic (HPLC) method with in-line connected photodiode-array (PDA) detection was developed and validated to identify and quantify nine B-ring unsubstituted and three B-ring substituted flavonoids in ten Australian species of Eucalyptus. Of these, eight compounds were detected and quantified in the crude methanolic extracts of leaves of various Eucalyptus species ( E. sieberi, E. rossii, E. fastigata, E. macrorhyncha, E. fraxinoides, E. agglomerata, E. consideniana, E. pauciflora, E. dives and E. obliqua) based on comparison with the retention times and λmax values of pure compounds. This rapid and sensitive HPLC/PDA method was coupled with electrospray ionization mass spectrometry (HPLC-ESI-MS) for qualitative analysis to corroborate the identification of compounds by HPLC/PDA analysis.

Quantitative Analysis of Various B-ring Unsubstituted and Substituted Flavonoids in Ten Australian Species of Eucalyptus