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A new C 23  steroid, (3β,5β,14β)-methyl (3-hydroxy-14,15-epoxy-20-oxo-21-norcholan-24-oate) (1), together with four known ones (2–5), were isolated from the venom of Bufo bufo gargarizans. Their structures were elucidated on the basis of extensive spectroscopic analysis. The cytotoxicity of these compounds was also evaluated against human hepatocarcinoma HepG2 cells. Compound 3 showed significant cytotoxicity with an IC 50  value of 16.8±0.7 μM.

A New C23 Steroid from the Venom of Bufo Bufo Gargarizans

A New C₂₃ Steroid from the Venom of Bufo Bufo Gargarizans