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A new apotirucallane-type triterpenoid with an unusual 22,23-epoxy group in the side chain, 22,23-epoxy-apotirucalla-14-ene-3α,7α,24α,25-tetraol (1), was isolated from the leaves and twigs of Orophea yunnanensis. The structure of 1 was established on the basis of HRESIMS, 1D, and 2D NMR spectroscopic methods. This is the first phytochemical study of Orophea yunnanensis, and the biogenetic origin of 1 was postulated. Compound 1 exhibited weak cytotoxicity in vitro against the growth of CNE1 nasopharyngeal carcinoma cell line with an IC 50  value of 9.7 μg/mL.

Oropheayunnol, an Unusual 22,23-Epoxy Apotirucallane Triterpenoid from Orophea Yunnanensis