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New steroid glycosides, hylodoside A (1) and novaeguinoside Y (2), along with previously known polyhydroxylated steroids (3-7) were isolated from the ethanolic extracts of the starfish Leptasterias hylodes reticulata and Culcita novaeguineae (juvenile). The structures have been elucidated using 1D and 2D NMR spectra ( 1 H,  13 C, DEPT, COSY-45, 1D-TOCSY, HSQCGP, ROESY, HSQC and HMBCGP) and mass spectrometry. Compound 3 was shown to inhibit the growth of Staphylococcus aureus up to 10% from the control at a concentration of 1 mg/mL. Steroids 1, 2 and 5 showed moderate hemolytic activity in the mouse erythrocytes assay. Compounds 3, 4 and 6 displayed pH-depended hemolytic properties.

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Two New Steroidal Saponins, Hylodoside A and Novaeguinoside Y, from the Starfish <i>Leptasterias Hylodes Reticulata</i> and <i>Culcita Novaeguineae</i> (Juvenile)

Two New Steroidal Saponins, Hylodoside A and Novaeguinoside Y, from the Starfish Leptasterias Hylodes Reticulata and Culcita Novaeguineae (Juvenile)