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The investigation of the steroidal glycosides of the seeds of Hyoscyamus niger L. led to the isolation of two new spirostane saponins (2, 6) together with seven known glycosides with either furostane or spirostane aglycones. On the basis of chemical and spectroscopic evidence, the structures of 2 and 6 were elucidated as (25 R)-5α-spirostan-3β-ol 3- O-β-D-glucopyranosyl-(1→3)-β-D-galactopyranoside (2) and (25 R)-5α-spirostan-3β-ol 3- O-β-D-glucopyranosyl-(1→3)-[β-D-glucopyranosyl-(1→2)]-β-D-galactopyranoside (6). The given compounds have been found for the first time in the genus Hyoscyamus.

Steroidal Glycosides from the Seeds of Hyoscyamus niger L