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The leaf essential oil of Eugenia sp. nov. “San Bosco” inhibits cruzain, a cysteine protease from Trypanosoma cruzi, the parasitic protozoan responsible for Chagas disease, with an IC 50  of 36.4 μg/mL. Eugenia “San Bosco” leaf oil is dominated by the sesquiterpene hydrocarbons zingiberene (24.7%) and germacrene D (11.1%), and these two compounds (IC 50  = 8.6 and 21.2 μg/mL, respectively) are likely responsible for the cruzain inhibitory activity observed in the essential oil.

Cruzain Inhibitory Activity of the Leaf Essential Oil from an Undescribed Species of Eugenia from Monteverde, Costa Rica