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A streamlined strategy was developed to obtain a mixture of thapsigargins from the fruits of Thapsia garganica L. The mixture was resolved by RP-silica gel flash chromatography to provide, after trituration with ether, 0.32% thapsigargin (1a) as a white powder. A novel, complex phenylpropanoid (neohelmanthicin D, 2a) was also obtained, and its structure confirmed by semisynthesis.

An Expeditious, Multi-gram Isolation Protocol for the Ultrapotent SERCA Inhibitor Thapsigargin