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The chemical content of outer birch bark extractive from the North American birches Betula papyrifera Marshall (paper birch) and B. neoalaskana Sargent (Alaskan paper birch) has been evaluated by GC/MS, HPLC and NMR spectroscopy. The comparative antiproliferative activity of the extractive and major triterpenoid components was studied using a number of human and murine malignant (P19, N2/D1, K1735-M2, PC-3 and CaOV3) and normal (BJ) cell lines. Betulin 3-caffeate was found to exhibit the highest antiproliferative activity among all triterpenoid components, including betulinic acid. Synthesis of betulin 3-caffeate from betulin has been developed.

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Analysis and Antiproliferative Activity of Bark Extractives of <i>Betula Neoalaskana</i> and <i>B. Papyrifera</i>. Synthesis of the Most Active Extractive Component - Betulin 3-Caffeate

Analysis and Antiproliferative Activity of Bark Extractives of Betula Neoalaskana and B. Papyrifera. Synthesis of the Most Active Extractive Component - Betulin 3-Caffeate