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In continuation of our search for anti-inflammatory constituents in lipophilic extracts of Isatis tinctoria, an HPLC-based profiling for inhibitors of human neutrophil elastase was performed. Using a linear gradient profile, fractions with significant activity (&gt;50%) appeared in the second half of the chromatogram and corresponded to moderately to highly lipophilic constituents. The active fractions of intermediate polarity were further analyzed by HPLC, TLC and GC. They contained mainly α-linolenic, linoleic, cis-11-octadecenoic acid, oleic and palmitic acids.

HPLC Based Activity Profiling for Inhibitors of Human Neutrophil Elastase in Isatis tinctoria Leaf Extracts