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The  13 C-mixed triacylglycerol (MTG) breath test is used to measure intraluminal fat digestion. In normal digestion 20% to 40% of the ingested  13 C label is recovered in breath CO 2 . We aimed to identify the proportions of ingested label excreted in stool as well as breath following ingestion of  13 C-MTG by children with impaired exocrine pancreatic function and healthy controls.  13 C enrichment of breath samples was measured by continuous flow isotope ratio mass spectrometry (IRMS) and the cumulative percent dose recovered (cPDR) in 10 hours was calculated. Total  13 C of a fecal fat extract from each stool was measured by combustion-IRMS, and  13 C enrichment and concentration of the tert.-butyldimethylsilyl (TBDMS) derivative of octanoic acid was measured by gas chromatography-mass spectrometry (GC/MS) after hydrolysis of the fat extract. Stool 5-day cPDR was calculated. Mean breath cPDR was 33% for children with cystic fibrosis and 45% for controls. Mean cPDR in stool by combustion-IRMS and GC/MS, respectively, was 0.7% and 0.3% for children with cystic fibrosis and 1.4 and 4.2% for controls.

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Bulk and Compound Specific Analysis of Stool Lipid Confirm that the “Missing” <sup>13</sup>C in the Mixed Triacylglycerol Breath Test is not in the Stool

Bulk and Compound Specific Analysis of Stool Lipid Confirm that the “Missing” 13C in the Mixed Triacylglycerol Breath Test is not in the Stool

Bulk and Compound Specific Analysis of Stool Lipid Confirm that the “Missing” ¹³C in the Mixed Triacylglycerol Breath Test is not in the Stool