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Kidney tissues from 20 cattle infected with Leptospira interrogans serovars hardjo, pomona, or grippotyphosa were cultured on the day of slaughter and 3, 6, and 8 days later to examine the effect of storage time on the recovery of leptospires by conventional culture methods. Leptospires were isolated from 85% of infected bovine kidney tissues cultured on day 1, and from 95%, 90%, and 90% of kidney tissues stored in transport medium at 4 C for 3, 6, and 8 days, respectively, prior to inoculation of culture media.

The Effect of Storage Time on Isolation of Leptospira Interrogans from Bovine Kidneys