Open AccessQuadruple Immunofluorescence: A Direct Visualization Method
Author(s) -
GianLuca Ferri,
Rosa Maria Gaudio,
Ida F. Castello,
Peter Berger,
G. Giro
Publication year - 1997
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/002215549704500201
Subject(s) - immunofluorescence , visualization , fluorescence , visible spectrum , chemistry , optics , computer science , physics , biology , antibody , artificial intelligence , immunology
We describe fluorescence immunostaining of four different antigens in the same section. The fluorochrome conjugates used show highest emission in the blue, green, yellow, and red regions of the visible light spectrum, respectively. Specially designed single fluorochrome filter combinations allow selective visualization of each fluorochrome label in turn, without visible crosstalk with the others.
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