Open AccessUltrathinfilm Immunoelectrophoresis
Author(s) -
Anne Michiak,
P M Clark,
Larry J. Kricka
Publication year - 1982
Publication title -
annals of clinical biochemistry international journal of laboratory medicine
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.6
H-Index - 80
eISSN - 1758-1001
pISSN - 0004-5632
DOI - 10.1177/000456328201900210
Subject(s) - immunoelectrophoresis , agarose , chromatography , reagent , chemistry , electrophoresis , albumin , medicine , biochemistry , immunology , antibody , organic chemistry
Immunoelectrophoresis (Laurell-rocket) in ultrathinfilm gels has been applied to the determination of human serum albumin in a model study. Agarose gels of 0.24 mm thickness were used and optimum electrophoretic conditions were determined. Precision was assessed and comparison with conventional immunoelectrophoresis and a chemical method was made. Though precision was poor (CV 4-6%) improvements in dispensing small volumes (0.2 microliter) should change this. Good correlation between methods (correlation coefficient 0.89-0.97) was obtained. The advantages of the technique are speed of analysis and low reagent costs.
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