English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

In pyruvate kinase deficiency the high activity of the leucocyte isoenzyme may mask the erythrocyte defect. Separation of leucocytes from erythrocytes by the commonly used sedimentation or filtration procedures requires rather large volumes of blood, is time-consuming, and sometimes leaves an unsatisfactorily large proportion of leucocytes. In the present study at least 95% of leucocytes, measured with the lysosomal enzyme β-N-acetylhexosaminidase as a very sensitive marker for leucocyte lysis, were consistently removed by differential lysis of erythrocytes in hypotonic saline. Haemolysates suitable for erythrocyte enzyme assays can be prepared from very small quantities of capillary blood (10–100 μl) very rapidly, cheaply, and reproducibly by this method.

Rapid Micromethod for the Preparation of Leucocyte-Free Haemolysates for the Determination of Pyruvate Kinase and other Erythrocyte Enzymes