English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background   The bromocresol green (BCG) assay is commonly used for measuring albumin (ALB), but is affected by α 1 - and α 2 -globulins, which are elevated in systemic inflammation. The modified bromocresol purple (mBCP) assay is another dye-binding method developed to overcome non-specific reactions. Concentrations of α 2 -macroglobulin, a major α 2 -globulin component, are increased in nephrotic syndrome (NS), but not in inflammation. There is little direct evidence that α 2 -macroglobulin affects BCG or mBCP assays.Methods   We measured serum albumin concentrations in 33 patients with NS and 13 reference healthy controls using BCG (ALB BCG ) and mBCP (ALB mBCP ) assays, and nephelometry (nALB) as a reference method. We also determined five specific proteins belonging to the α 1 - and α 2 -globulins by nephelometry. After adding purified α 2 -macroglobulin to albumin solutions, protein reactivity in these three assays was compared.Results   Nephrotic syndrome patients were categorized to tertiles according to nALB concentration. In all tertiles, ALB BCG  was significantly higher than nALB, especially in the severe hypoalbuminemia group, in which α 2 -macroglobulin was 43–49% higher. By contrast, ALB mBCP  and nALB were almost identical in all three groups. The difference between ALB BCG  and nALB was positively correlated with the α 2 -macroglobulin concentration. In vitro, when α 2 -macroglobulin was added to solutions containing identical albumin concentrations, α 2 -macroglobulin dose-dependently increased ALB BCG , but not ALB mBCP .Conclusions   In NS, α 2 -macroglobulin is a major factor for positive bias of ALB BCG , especially in patients with severe hypoalbuminemia. The mBCP assay is useful for measuring albumin concentrations in NS.

annals of clinical biochemistry

The bromocresol green assay, but not the modified bromocresol purple assay, overestimates the serum albumin concentration in nephrotic syndrome through reaction with α<sub>2</sub>-macroglobulin

The bromocresol green assay, but not the modified bromocresol purple assay, overestimates the serum albumin concentration in nephrotic syndrome through reaction with α2-macroglobulin

The bromocresol green assay, but not the modified bromocresol purple assay, overestimates the serum albumin concentration in nephrotic syndrome through reaction with α₂-macroglobulin