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Parathyroid hormone (PTH) concentrations were compared in serum and EDTA plasma from 36 patients attending a renal stone clinic. Serum PTH concentrations ranged from 0·9 to 10·9pmol/L, with a mean of 4·6pmol/L. When serum and EDTA plasma results were compared, in samples frozen within 30 min of collection, EDTA plasma results were found to be significantly higher than those in serum ( P &lt;0·0001; Wilcoxon test), with an average increase of 19·5% over the serum result. Results from EDTA-preserved blood left to stand at room temperature for 48 h were on average 14·8% lower than results from the corresponding EDTA plasma samples frozen within 30 min, with a highly significant difference ( P &lt;0·0001). Freshly frozen serum and 48h EDTA plasma PTH results were not significantly different.    Parathyroid hormone in EDTA-preserved blood is not completely stable, and this could lead to misclassification of results for samples which are not frozen quickly.

Immulite 2000 Parathyroid Hormone Assay: Stability of Parathyroid Hormone in EDTA Blood Kept at Room Temperature for 48 h