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time that the red cells of patients with paroxysmal nocturnal hemoglobinuria (PNH) are unusually susceptible to lysis by hetero- and isoantibodies and complement. These observations have since been confirmed by many other studies using a variety of antibodies (2). The susceptibility of these cells to lysis by antibody and complement has been utilized in the detection of hemolytic antibodies in human serum (3) and in the analysis of the kinetics of hemolysis of human cells by specific antibody and complement (4). The red cells of patients with PNH are also lysed by fresh normal serum, especially when the pH is reduced to 6.5 to 7.0 (5, 6). This reaction, the acidified serum test or Ham's test, is of major importance in the diagnosis of the disease. Lysis by acidified normal serum is abolished when the serum is treated with ammonia or heated to 56° C

the journal of clinical investigation/the journal of clinical investigation

Immune lysis of normal human and paroxysmal nocturnal hemoglobinuria (PNH) red blood cells. I. The sensitivity of PNH red cells to lysis by complement and specific antibody.