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The preparation of I131-labeled human insulin from a lot of human insulin containing approximately 25% insulin by weight and its purification from labeled contaminants are described. The reaction of human insulin-I133 with insulinbinding antibodies in the serums of human subjects treated with commercial mixtures of animal insulins is demonstrated directly. Comparison of the binding of human insulin and beef insulin in antiserums from eight insulinresistant and nonresistant diabetic subjects revealed a lesser affinity' of antibody for human than for beef insulin in most cases, but considerable variability in this respect was encountered among different antiserums.

PREPARATION AND PURIFICATION OF HUMAN INSULIN-I131; BINDING TO HUMAN INSULIN-BINDING ANTIBODIES